› 5sx5

X-ray diffraction
2.5Å resolution

Crystal Structure of panitumumab in complex with epidermal growth factor receptor domain 3 mutant S468R.

Released:
DOI: 10.2210/pdb5sx5/pdb
PDB entry 5sx5 coloured by chain, front view.
PDB entry 5sx5 coloured by chain, side view.
PDB entry 5sx5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Panitumumab EGFR Complex Reveals a Binding Mechanism That Overcomes Cetuximab Induced Resistance.
Sickmier EA, Kurzeja RJ, Michelsen K, Vazir M, Yang E, Tasker AS
PLoS One 11 e0163366
PMID: 27658254

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230713 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Panitumumab Fab Light Chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 214 amino acids
Theoretical weight: 23.38 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Panitumumab Fab Heavy Chain Chains: H, J
Molecule details ›
Chains: H, J
Length: 221 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chains: M, N
Molecule details ›
Chains: M, N
Length: 201 amino acids
Theoretical weight: 22.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 335-525; Coverage: 16%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Receptor L domain
Structure domains: Receptor L-domain

Ligands and Environments

4 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 4 x GOL
Ligand EDO 3 x EDO
Ligand 1PE 3 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 65.073Å b: 113.106Å c: 231.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.25
Expression systems:
  • Cricetulus griseus
  • Spodoptera frugiperda

Quick links

Citations

16 review citations

Monoclonal Antibodies in Cancer Therapy.
Zahavi et al. (2020)
15 more

1 mention without citation

Breaking barriers in antibody discovery: harnessing divergent species for accessing difficult and conserved drug targets.
Banik et al. (2023)