› 5sxu

X-ray diffraction
3.1Å resolution

X-ray structure of 2-bromoethanol bound to a pentameric ligand gated ion channel (ELIC) in a desensitized state

Released:
DOI: 10.2210/pdb5sxu/pdb
PDB entry 5sxu coloured by chain, front view.
PDB entry 5sxu coloured by chain, side view.
PDB entry 5sxu coloured by chain, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion Channel ELIC.
Chen Q, Wells MM, Tillman TS, Kinde MN, Cohen A, Xu Y, Tang P
Structure 25 180-187 (2017)
PMID: 27916519

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 322 amino acids
Theoretical weight: 36.88 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 22-343; Coverage: 100%)
Gene name: Dda3937_00520
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BRJ 32 x BRJ
Ligand 3CN 10 x 3CN
Ligand MES 10 x MES
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 105.903Å b: 266.791Å c: 111.109Å
α: 90° β: 107.13° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent progress on the molecular pharmacology of propofol.
Tang et al. (2018)
5 other articles

2 mentions without citation

Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion Channel ELIC.
Chen et al. (2017)
1 more