› 5syd

X-ray diffraction
2.4Å resolution

Circularly permutated azurin (cpAz) based on P. aeruginosa azurin sequence

Released:
DOI: 10.2210/pdb5syd/pdb
PDB entry 5syd coloured by chain, front view.
PDB entry 5syd coloured by chain, side view.
PDB entry 5syd coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Petrik I, Yang Y, Howard R, Yi L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.08 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 63-148; Coverage: 67%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 115.436Å b: 115.436Å c: 83.145Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.224 0.251
Expression system: Escherichia coli BL21(DE3)

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