› 5t00

X-ray diffraction
2.19Å resolution

Human CTCF ZnF3-7 and methylated DNA complex

Released:
DOI: 10.2210/pdb5t00/pdb
PDB entry 5t00 coloured by chain, front view.
PDB entry 5t00 coloured by chain, side view.
PDB entry 5t00 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for the Versatile and Methylation-Dependent Binding of CTCF to DNA.
Hashimoto H, Wang D, Horton JR, Zhang X, Corces VG, Cheng X
Mol Cell 66 711-720.e3 (2017)
PMID: 28529057

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119127 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor CTCF Chains: A, D
Molecule details ›
Chains: A, D
Length: 150 amino acids
Theoretical weight: 17.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49711 (Residues: 321-465; Coverage: 20%)
Gene name: CTCF
Sequence domains: Zinc finger, C2H2 type
DNA (5'-TAG(5CM)GCCCCCTGCTGGC-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-GCCAGCAGGGGG(5CM)GCTA-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 17 nucleotides
Theoretical weight: 5.29 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 10 x ZN
1 modified residue:
Ligand 5CM 4 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 40.99Å b: 44.91Å c: 86.8Å
α: 98.33° β: 92.4° γ: 94.8°
R-values:
R R work R free
0.22 0.218 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

26 review citations

Dynamic DNA methylation: In the right place at the right time.
Luo et al. (2018)
25 more

9 mentions without citation

Zinc Finger Readers of Methylated DNA.
Hudson et al. (2018)
8 more