› 5t09

X-ray diffraction
2.01Å resolution

The structure of the type III effector HopBA1

Released:
DOI: 10.2210/pdb5t09/pdb
PDB entry 5t09 coloured by chain, front view.
PDB entry 5t09 coloured by chain, side view.
PDB entry 5t09 coloured by chain, top view.
Source organism: Pseudomonas syringae pv. aptata
Primary publication:
TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis.
Nishimura MT, Anderson RG, Cherkis KA, Law TF, Liu QL, Machius M, Nimchuk ZL, Yang L, Chung EH, El Kasmi F, Hyunh M, Osborne Nishimura E, Sondek JE, Dangl JL
Proc Natl Acad Sci U S A 114 E2053-E2062 (2017)
PMID: 28137883

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type III effector HopBA1 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.68 KDa
Source organism: Pseudomonas syringae pv. aptata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0Q0CD50 (Residues: 1-239; Coverage: 100%)
Gene names: ALO85_00915, ALQ37_101512

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 64.865Å b: 64.865Å c: 96.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

What Do We Know About NOD-Like Receptors in Plant Immunity?
Zhang et al. (2017)
14 more

4 mentions without citation

α-Helices in the Type III Secretion Effectors: A Prevalent Feature with Versatile Roles.
Gazi et al. (2021)
3 more