› 5t2f

X-ray diffraction
2.66Å resolution

Structure of the FHA1 domain of Rad53 bound to the BRCT domain of Dbf4

Released:
DOI: 10.2210/pdb5t2f/pdb
Model geometry
Fit model/data
PDB entry 5t2f coloured by chain, front view.
PDB entry 5t2f coloured by chain, side view.
PDB entry 5t2f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
'AND' logic gates at work: Crystal structure of Rad53 bound to Dbf4 and Cdc7.
Almawi AW, Matthews LA, Myrox P, Boulton S, Lai C, Moraes T, Melacini G, Ghirlando R, Duncker BP, Guarné A
OpenAccess logo Sci Rep 6 34237 (2016)
PMID: 27681475

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DDK kinase regulatory subunit DBF4; Serine/threonine-protein kinase RAD53 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 269 amino acids
Theoretical weight: 30.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32325 (Residues: 105-220; Coverage: 17%)
  • Canonical: P22216 (Residues: 22-162; Coverage: 17%)
Gene names: D4205, DBF4, DNA52, MEC2, P2588, RAD53, SAD1, SPK1, YD9609.07C, YDR052C, YPL153C
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 14 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 57.775Å b: 66.527Å c: 86.52Å
α: 109.24° β: 90.88° γ: 90.3°
R-values:
R R work R free
0.208 0.206 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

FHA domains: Phosphopeptide binding and beyond.
Almawi et al. (2017)
2 more

3 mentions without citation

'AND' logic gates at work: Crystal structure of Rad53 bound to Dbf4 and Cdc7.
Almawi et al. (2016)
2 more