› 5t46

X-ray diffraction
1.53Å resolution

Crystal structure of the human eIF4E-eIF4G complex

Released:
DOI: 10.2210/pdb5t46/pdb
PDB entry 5t46 coloured by chain, front view.
PDB entry 5t46 coloured by chain, side view.
PDB entry 5t46 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation.
Grüner S, Peter D, Weber R, Wohlbold L, Chung MY, Weichenrieder O, Valkov E, Igreja C, Izaurralde E
Mol Cell 64 467-479 (2016)
PMID: 27773676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139175 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chains: A, C
Molecule details ›
Chains: A, C
Length: 220 amino acids
Theoretical weight: 25.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4 gamma 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 66 amino acids
Theoretical weight: 7.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04637 (Residues: 592-653; Coverage: 4%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

2 bound ligands:
Ligand MGP 2 x MGP
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 45.25Å b: 70.347Å c: 79.913Å
α: 90° β: 99.37° γ: 90°
R-values:
R R work R free
0.14 0.139 0.162
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Protein Synthesis Initiation in Eukaryotic Cells.
Merrick et al. (2018)
18 more

7 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
6 more