› 5t48

X-ray diffraction
2.19Å resolution

Crystal structure of the D. melanogaster eIF4E-eIF4G complex without lateral contact

Released:
DOI: 10.2210/pdb5t48/pdb
Model geometry
Fit model/data
PDB entry 5t48 coloured by chain, front view.
PDB entry 5t48 coloured by chain, side view.
PDB entry 5t48 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation.
Grüner S, Peter D, Weber R, Wohlbold L, Chung MY, Weichenrieder O, Valkov E, Igreja C, Izaurralde E
Mol Cell 64 467-479 (2016)
PMID: 27773676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130238 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E1 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 21.25 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48598 (Residues: 80-259; Coverage: 70%)
Gene names: CG4035, Eif4e, eIF-4E, eIF4E1
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4G1, isoform A Chain: B
Molecule details ›
Chain: B
Length: 64 amino acids
Theoretical weight: 7.41 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O61380 (Residues: 601-660; Coverage: 4%)
Gene names: CG10811, Dmel\CG10811, Dmel_CG10811, EIF4G, EIF4G1, Eif4G, deIF4G, eIF-4G, eIF4G, eIF4G-RA, eIF4G1, eIF4g, eiF-4G, p200
Sequence domains: Translation initiation factor eIF4G-like, eIF4E-binding domain

Ligands and Environments

1 bound ligand:
Ligand MGP 1 x MGP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 45.622Å b: 66.196Å c: 75.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Protein Synthesis Initiation in Eukaryotic Cells.
Merrick et al. (2018)
20 more