› 5t5j

X-ray diffraction
1.35Å resolution

LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH TN-PEPTIDE

Released:
DOI: 10.2210/pdb5t5j/pdb
PDB entry 5t5j coloured by chain, front view.
PDB entry 5t5j coloured by chain, side view.
PDB entry 5t5j coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.
Lubkowski J, Durbin SV, Silva MC, Farnsworth D, Gildersleeve JC, Oliva ML, Wlodawer A
FEBS J 284 429-450 (2017)
PMID: 27973758

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160990 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.14 KDa
Source organism: Bauhinia forficata
Expression system: Escherichia coli
UniProt:
  • Canonical: P86993 (Residues: 1-233; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls
TN ANTIGEN ACA-SER-SER-VAL-GLY Chains: a, b
Molecule details ›
Chains: a, b
Length: 5 amino acids
Theoretical weight: 462 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand EDO 7 x EDO
Ligand A2G 2 x A2G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 54.378Å b: 46.159Å c: 92.107Å
α: 90° β: 92.56° γ: 90°
R-values:
R R work R free
0.106 0.105 0.141
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Plant-Derived Lectins as Potential Cancer Therapeutics and Diagnostic Tools.
Mazalovska et al. (2020)
4 more

2 mentions without citation

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy.
Poiroux et al. (2017)
1 more