› 5t5w

X-ray diffraction
2.85Å resolution

Structure of an affinity matured lambda-IFN/IFN-lambdaR1/IL-10Rbeta receptor complex

Released:
DOI: 10.2210/pdb5t5w/pdb
PDB entry 5t5w coloured by chain, front view.
PDB entry 5t5w coloured by chain, side view.
PDB entry 5t5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The IFN-λ-IFN-λR1-IL-10Rβ Complex Reveals Structural Features Underlying Type III IFN Functional Plasticity.
Mendoza JL, Schneider WM, Hoffmann HH, Vercauteren K, Jude KM, Xiong A, Moraga I, Horton TM, Glenn JS, de Jong YP, Rice CM, Garcia KC
Immunity 46 379-392 (2017)
PMID: 28329704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-170634 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-10 receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 25.05 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q08334 (Residues: 19-220; Coverage: 66%)
Gene names: CRFB4, D21S58, D21S66, IL10RB
Sequence domains:
Structure domains: Immunoglobulins
Interferon lambda receptor 1 Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IU57 (Residues: 21-226; Coverage: 41%)
Gene names: IFNLR1, IL28RA, LICR2
Sequence domains: Tissue factor
Structure domains: Immunoglobulins
Interferon lambda-3 Chain: C
Molecule details ›
Chain: C
Length: 176 amino acids
Theoretical weight: 19.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8IZI9 (Residues: 34-195; Coverage: 93%)
Gene names: IFNL3, IL28B, IL28C, ZCYTO22
Sequence domains: Interleukin-28A

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3121
Unit cell:
a: 106.79Å b: 106.79Å c: 129.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.253
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

20 review citations

Shared and Distinct Functions of Type I and Type III Interferons.
Lazear et al. (2019)
19 more

18 mentions without citation

The Role of Structure in the Biology of Interferon Signaling.
Walter MR. (2020)
17 more