› 5t6o

X-ray diffraction
1.8Å resolution

Structure of the catalytic domain of the class I polyhydroxybutyrate synthase from Cupriavidus necator

Released:
DOI: 10.2210/pdb5t6o/pdb
PDB entry 5t6o coloured by chain, front view.
PDB entry 5t6o coloured by chain, side view.
PDB entry 5t6o coloured by chain, top view.
Source organism: Cupriavidus necator
Primary publication:
Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator.
Wittenborn EC, Jost M, Wei Y, Stubbe J, Drennan CL
J Biol Chem 291 25264-25277 (2016)
PMID: 27742839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(3-hydroxyalkanoate) polymerase subunit PhaC Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.64 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli
UniProt:
  • Canonical: P23608 (Residues: 201-589; Coverage: 66%)
Gene names: H16_A1437, phaC, phbC
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 72.08Å b: 87.82Å c: 137.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.2
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications.
Wong et al. (2020)
7 more

3 mentions without citation

Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator.
Wittenborn et al. (2016)
2 more