› 5t77

X-ray diffraction
2Å resolution

Crystal structure of the MOP flippase MurJ

Released:
DOI: 10.2210/pdb5t77/pdb
PDB entry 5t77 coloured by chain, front view.
PDB entry 5t77 coloured by chain, side view.
PDB entry 5t77 coloured by chain, top view.
Source organism: Thermosipho africanus TCF52B
Primary publication:
Crystal structure of the MOP flippase MurJ in an inward-facing conformation.
Kuk AC, Mashalidis EH, Lee SY
Nat Struct Mol Biol 24 171-176 (2017)
PMID: 28024149

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid II flippase MurJ Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 54.14 KDa
Source organism: Thermosipho africanus TCF52B
Expression system: Escherichia coli
UniProt:
  • Canonical: B7IE18 (Residues: 1-475; Coverage: 100%)
Gene names: THA_1814, murJ, mviN
Sequence domains: Lipid II flippase MurJ

Ligands and Environments

7 bound ligands:
Ligand CL 5 x CL
Ligand ZN 1 x ZN
Ligand CA 3 x CA
Ligand NA 2 x NA
Ligand 1PE 3 x 1PE
Ligand OLB 7 x OLB
Ligand OLC 32 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 94.163Å b: 99.573Å c: 74.66Å
α: 90° β: 112.91° γ: 90°
R-values:
R R work R free
0.19 0.189 0.214
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
11 more

12 mentions without citation

Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.
Chamakura et al. (2019)
11 more