› 5t7t

X-ray diffraction
1.96Å resolution

Galectin-8 N terminal domain in complex with LNT

Released:
DOI: 10.2210/pdb5t7t/pdb
PDB entry 5t7t coloured by chain, front view.
PDB entry 5t7t coloured by chain, side view.
PDB entry 5t7t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based rationale for differential recognition of lacto- and neolacto- series glycosphingolipids by the N-terminal domain of human galectin-8.
Bohari MH, Yu X, Zick Y, Blanchard H
Sci Rep 6 39556 (2016)
PMID: 28000747

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-8 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00214 (Residues: 1-155; Coverage: 49%)
Gene name: LGALS8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 45.4Å b: 49.61Å c: 80.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.215
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Multifunctional Benefits of Prevalent HMOs: Implications for Infant Health.
Hill et al. (2021)
4 other articles

2 mentions without citation

Conformations of the type-1 lacto-N-biose I unit in protein complex structures.
Fushinobu S. (2018)
1 more