› 5t7u

X-ray diffraction
1.58Å resolution

Crystal structure of galectin-8N in complex with Glycerol

Released:
DOI: 10.2210/pdb5t7u/pdb
PDB entry 5t7u coloured by chain, front view.
PDB entry 5t7u coloured by chain, side view.
PDB entry 5t7u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based rationale for differential recognition of lacto- and neolacto- series glycosphingolipids by the N-terminal domain of human galectin-8.
Bohari MH, Yu X, Zick Y, Blanchard H
Sci Rep 6 39556 (2016)
PMID: 28000747

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galectin-8 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00214 (Residues: 1-155; Coverage: 49%)
Gene name: LGALS8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand NA 1 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 47.4Å b: 50.3Å c: 69.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.118 0.147
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Multifunctional Benefits of Prevalent HMOs: Implications for Infant Health.
Hill et al. (2021)
1 more

1 mention without citation

Structure-based rationale for differential recognition of lacto- and neolacto- series glycosphingolipids by the N-terminal domain of human galectin-8.
Bohari et al. (2016)