› 5t80

X-ray diffraction
2.62Å resolution

Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidic acid (06:0 PA)

Released:
DOI: 10.2210/pdb5t80/pdb
PDB entry 5t80 coloured by chain, front view.
PDB entry 5t80 coloured by chain, side view.
PDB entry 5t80 coloured by chain, top view.
Source organisms:
Primary publication:
Lipid interactions and angle of approach to the HIV-1 viral membrane of broadly neutralizing antibody 10E8: Insights for vaccine and therapeutic design.
Irimia A, Serra AM, Sarkar A, Jacak R, Kalyuzhniy O, Sok D, Saye-Francisco KL, Schiffner T, Tingle R, Kubitz M, Adachi Y, Stanfield RL, Deller MC, Burton DR, Schief WR, Wilson IA
PLoS Pathog 13 e1006212 (2017)
PMID: 28225819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-230984 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody 10E8 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody 10E8 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 22.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
10E8 EPITOPE SCAFFOLD T117V2 Chain: G
Molecule details ›
Chain: G
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

1 bound ligand:
Ligand 44E 1 x 44E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 55.221Å b: 67.262Å c: 196.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.243
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

7 review citations

Recent progress in broadly neutralizing antibodies to HIV.
Sok et al. (2018)
6 more

5 mentions without citation

Lipid interactions and angle of approach to the HIV-1 viral membrane of broadly neutralizing antibody 10E8: Insights for vaccine and therapeutic design.
Irimia et al. (2017)
4 more