› 5tcy

X-ray diffraction
1.9Å resolution

A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with CeuE (H227L variant), a periplasmic protein from Campylobacter jejuni.

Released:
DOI: 10.2210/pdb5tcy/pdb
PDB entry 5tcy coloured by chain, front view.
PDB entry 5tcy coloured by chain, side view.
PDB entry 5tcy coloured by chain, top view.
Source organism: Campylobacter jejuni BJ-CJGB96299
Primary publication:
Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length.
Wilde EJ, Hughes A, Blagova EV, Moroz OV, Thomas RP, Turkenburg JP, Raines DJ, Duhme-Klair AK, Wilson KS
Sci Rep 7 45941 (2017)
PMID: 28383577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 291 amino acids
Theoretical weight: 32.06 KDa
Source organism: Campylobacter jejuni BJ-CJGB96299
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 44-330; Coverage: 93%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

2 bound ligands:
Ligand FE 3 x FE
Ligand 5LC 3 x 5LC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 58.696Å b: 62.881Å c: 69.867Å
α: 82.09° β: 74.67° γ: 77.6°
R-values:
R R work R free
0.174 0.171 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.
Zhang et al. (2020)
1 more

1 mention without citation

Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length.
Wilde et al. (2017)