5tda

X-ray diffraction
0.79Å resolution

Crystal structure of the UBR-box domain from UBR2 in complex with RLWS N-degron

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-166106 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UBR2 Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWV8 (Residues: 98-168; Coverage: 4%)
Gene names: C6orf133, KIAA0349, UBR2
Sequence domains: Putative zinc finger in N-recognin (UBR box)
ARG-LEU-TRP-SER peptide Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 562 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 28.702Å b: 37.158Å c: 57.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.12 0.128
Expression system: Escherichia coli