› 5te4

X-ray diffraction
2.75Å resolution

Crystal Structure of Broadly Neutralizing VRC01-class Antibody N6 in Complex with HIV-1 Clade G Strain X2088 gp120 Core

Released:
DOI: 10.2210/pdb5te4/pdb
Model geometry
Fit model/data
PDB entry 5te4 coloured by chain, front view.
PDB entry 5te4 coloured by chain, side view.
PDB entry 5te4 coloured by chain, top view.
Source organisms:
Primary publication:
Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth.
Huang J, Kang BH, Ishida E, Zhou T, Griesman T, Sheng Z, Wu F, Doria-Rose NA, Zhang B, McKee K, O'Dell S, Chuang GY, Druz A, Georgiev IS, Schramm CA, Zheng A, Joyce MG, Asokan M, Ransier A, Darko S, Migueles SA, Bailer RT, Louder MK, Alam SM, Parks R, Kelsoe G, Von Holle T, Haynes BF, Douek DC, Hirsch V, Seaman MS, Shapiro L, Mascola JR, Kwong PD, Connors M
Immunity 45 1108-1121 (2016)
PMID: 27851912

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207737 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 360 amino acids
Theoretical weight: 40.19 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: C6ZIH2 (Residues: 43-486; Coverage: 42%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
Heavy chain of antibody N6 Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Light chain of antibody N6 Chain: L

Ligands and Environments

4 bound ligands:
Ligand NAG 12 x NAG
Ligand SO4 8 x SO4
Ligand BU3 1 x BU3
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 64.764Å b: 72.227Å c: 218.123Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.267
Expression system: Homo sapiens

Quick links

Citations

59 review citations

Recent progress in broadly neutralizing antibodies to HIV.
Sok et al. (2018)
58 more

2 mentions without citation

Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth.
Huang et al. (2016)
1 more