› 5tfv

X-ray diffraction
2.54Å resolution

Crystal Structure of MT-I isolated from Bothrops asper venom.

Released:
DOI: 10.2210/pdb5tfv/pdb
PDB entry 5tfv coloured by chain, front view.
PDB entry 5tfv coloured by chain, side view.
PDB entry 5tfv coloured by chain, top view.
Source organism: Bothrops asper
Primary publication:
Crystal structure of a phospholipase A2 from Bothrops asper venom: Insights into a new putative "myotoxic cluster".
Salvador GH, Dos Santos JI, Lomonte B, Fontes MR
Biochimie 133 95-102 (2017)
PMID: 28034717

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 myotoxin I Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.99 KDa
Source organism: Bothrops asper
UniProt:
  • Canonical: P20474 (Residues: 17-138; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P3221
Unit cell:
a: 56.534Å b: 56.534Å c: 136.459Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.263

Quick links

Citations

2 review citations

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins.
Rivera-de-Torre et al. (2021)
1 more

6 mentions without citation

The chemistry of snake venom and its medicinal potential.
Oliveira et al. (2022)
5 more