› 5tgd

X-ray diffraction
1.7Å resolution

Crystal structure of FolM Alternative dihydrofolate reductase 1 from Brucella suis in complex with NADP

Released:
DOI: 10.2210/pdb5tgd/pdb
PDB entry 5tgd coloured by chain, front view.
PDB entry 5tgd coloured by chain, side view.
PDB entry 5tgd coloured by chain, top view.
Source organism: Brucella suis 92/29
Primary publication:
Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis.
Porter I, Neal T, Walker Z, Hayes D, Fowler K, Billups N, Rhoades A, Smith C, Smith K, Staker BL, Dranow DM, Mayclin SJ, Subramanian S, Edwards TE, Myler PJ, Asojo OA
Acta Crystallogr F Struct Biol Commun 78 31-38 (2022)
PMID: 34981773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FolM Alternative dihydrofolate reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 29.69 KDa
Source organism: Brucella suis 92/29
Expression system: Escherichia coli BL21(DE3)
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAP 4 x NAP
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 76.35Å b: 76.52Å c: 98.26Å
α: 90° β: 109.47° γ: 90°
R-values:
R R work R free
0.163 0.163 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis.
Porter et al. (2022)