› 5tha

X-ray diffraction
1.8Å resolution

Gemin5 WD40 repeats in complex with a guanosyl moiety

Released:
DOI: 10.2210/pdb5tha/pdb
PDB entry 5tha coloured by chain, front view.
PDB entry 5tha coloured by chain, side view.
PDB entry 5tha coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J
Genes Dev 30 2376-2390 (2016)
PMID: 27881600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gem-associated protein 5 Chain: A
Molecule details ›
Chain: A
Length: 758 amino acids
Theoretical weight: 84.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TEQ6 (Residues: 1-739; Coverage: 49%)
Gene name: GEMIN5
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand UNX 21 x UNX
Ligand GP3 1 x GP3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 58.198Å b: 125.253Å c: 60.889Å
α: 90° β: 117.1° γ: 90°
R-values:
R R work R free
0.169 0.167 0.2
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Deciphering the mRNP Code: RNA-Bound Determinants of Post-Transcriptional Gene Regulation.
Gehring et al. (2017)
6 more

2 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
1 more