› 5tim

X-ray diffraction
1.83Å resolution

REFINED 1.83 ANGSTROMS STRUCTURE OF TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE, CRYSTALLIZED IN THE PRESENCE OF 2.4 M-AMMONIUM SULPHATE. A COMPARISON WITH THE STRUCTURE OF THE TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE-GLYCEROL-3-PHOSPHATE COMPLEX

Released:
DOI: 10.2210/pdb5tim/pdb
PDB entry 5tim coloured by chain, front view.
PDB entry 5tim coloured by chain, side view.
PDB entry 5tim coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Refined 1.83 A structure of trypanosomal triosephosphate isomerase crystallized in the presence of 2.4 M-ammonium sulphate. A comparison with the structure of the trypanosomal triosephosphate isomerase-glycerol-3-phosphate complex.
Wierenga RK, Noble ME, Vriend G, Nauche S, Hol WG
J Mol Biol 220 995-1015 (1991)
PMID: 1880808

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 26.87 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Not provided
UniProt:
  • Canonical: P04789 (Residues: 1-250; Coverage: 100%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 113.14Å b: 97.69Å c: 46.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

Evolution of glycolysis.
Fothergill-Gilmore et al. (1993)
8 more

28 mentions without citation

Enzyme architecture: on the importance of being in a protein cage.
Richard et al. (2014)
27 more