› 5tjr

X-ray diffraction
2.95Å resolution

X-ray Crystal structure of a methylmalonate semialdehyde dehydrogenase from Pseudomonas sp. AAC

Released:
DOI: 10.2210/pdb5tjr/pdb
PDB entry 5tjr coloured by chain, front view.
PDB entry 5tjr coloured by chain, side view.
PDB entry 5tjr coloured by chain, top view.
Source organism: Pseudomonas sp. AAC
Primary publication:
Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida.
Tararina MA, Janda KD, Allen KN
Biochemistry 55 6595-6598 (2016)
PMID: 27933790

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylmalonate-semialdehyde dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 531 amino acids
Theoretical weight: 57.34 KDa
Source organism: Pseudomonas sp. AAC
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 115.891Å b: 156.631Å c: 192.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The enzymes of microbial nicotine metabolism.
Fitzpatrick PF. (2018)
1 more

1 mention without citation

X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC.
Wilding et al. (2017)