› 5tkr

X-ray diffraction
1.8Å resolution

Crystal structure of a Lipomyces starkeyi levoglucosan kinase G359R mutant

Released:
DOI: 10.2210/pdb5tkr/pdb
PDB entry 5tkr coloured by chain, front view.
PDB entry 5tkr coloured by chain, side view.
PDB entry 5tkr coloured by chain, top view.
Source organism: Lipomyces starkeyi
Primary publication:
Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning.
Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
Proc Natl Acad Sci U S A 114 2265-2270 (2017)
PMID: 28196882

Function and Biology Details

Reaction catalysed: 
ATP + levoglucosan + H(2)O = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levoglucosan kinase Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.59 KDa
Source organism: Lipomyces starkeyi
Expression system: Escherichia coli
UniProt:
  • Canonical: B3VI55 (Residues: 1-439; Coverage: 100%)
Sequence domains: Anhydro-N-acetylmuramic acid kinase

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand SO4 1 x SO4
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P41212
Unit cell:
a: 70.06Å b: 70.06Å c: 261.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Multiplexed assays of variant effects contribute to a growing genotype-phenotype atlas.
Weile et al. (2018)
17 more

1 mention without citation

Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning.
Klesmith et al. (2017)