› 5tnm

X-ray diffraction
1.7Å resolution

Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (R)-1,2-Epoxyoctane hydrolysis intermediate

Released:
DOI: 10.2210/pdb5tnm/pdb
PDB entry 5tnm coloured by chain, front view.
PDB entry 5tnm coloured by chain, side view.
PDB entry 5tnm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase.
Hvorecny KL, Bahl CD, Kitamura S, Lee KSS, Hammock BD, Morisseau C, Madden DR
Structure 25 697-707.e4 (2017)
PMID: 28392259

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 34.16 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZD27 (Residues: 25-319; Coverage: 100%)
Gene name: PA14_26090
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 7F2 4 x 7F2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: C2
Unit cell:
a: 168.297Å b: 84.043Å c: 89.253Å
α: 90° β: 100.39° γ: 90°
R-values:
R R work R free
0.147 0.146 0.171
Expression system: Escherichia coli

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Citations

1 review citation

Bacterial Outer Membrane Vesicles and Immune Modulation of the Host.
Charpentier et al. (2023)
7 other articles

2 mentions without citation

Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase.
Hvorecny et al. (2017)
1 more