› 5toj

X-ray diffraction
3.3Å resolution

Crystal structure of the RSV F glycoprotein in complex with the neutralizing single-domain antibody F-VHH-4

Released:
DOI: 10.2210/pdb5toj/pdb
PDB entry 5toj coloured by chain, front view.
PDB entry 5toj coloured by chain, side view.
PDB entry 5toj coloured by chain, top view.
Source organisms:
Primary publication:
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state.
Rossey I, Gilman MS, Kabeche SC, Sedeyn K, Wrapp D, Kanekiyo M, Chen M, Mas V, Spitaels J, Melero JA, Graham BS, Schepens B, McLellan JS, Saelens X
Nat Commun 8 14158 (2017)
PMID: 28194013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230767 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fusion glycoprotein F0 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 550 amino acids
Theoretical weight: 61.05 KDa
Source organism: Human orthopneumovirus
Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 1-516; Coverage: 89%)
Gene name: F
Sequence domains: Fusion glycoprotein F0
Single-domain antibody F-VHH-4 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 131 amino acids
Theoretical weight: 14.44 KDa
Source organism: Lama glama
Expression system: Komagataella phaffii GS115
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3221
Unit cell:
a: 173.189Å b: 173.189Å c: 153.301Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.234
Expression systems:
  • Homo sapiens
  • Komagataella phaffii GS115

Quick links

Citations

17 review citations

Passive immunotherapy of viral infections: 'super-antibodies' enter the fray.
Walker et al. (2018)
16 more

4 mentions without citation

Antibody Epitopes of Pneumovirus Fusion Proteins.
Huang et al. (2019)
3 more