› 5tp3

X-ray diffraction
1.87Å resolution

Crystal structure of the RSV-neutralizing single-domain antibody F-VHH-4

Released:
DOI: 10.2210/pdb5tp3/pdb
PDB entry 5tp3 coloured by chain, front view.
PDB entry 5tp3 coloured by chain, side view.
PDB entry 5tp3 coloured by chain, top view.
Source organism: Lama glama
Primary publication:
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state.
Rossey I, Gilman MS, Kabeche SC, Sedeyn K, Wrapp D, Kanekiyo M, Chen M, Mas V, Spitaels J, Melero JA, Graham BS, Schepens B, McLellan JS, Saelens X
Nat Commun 8 14158 (2017)
PMID: 28194013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F-VHH-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.61 KDa
Source organism: Lama glama
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 47.71Å b: 47.77Å c: 149.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.17 0.208
Expression system: Homo sapiens

Quick links

Citations

18 review citations

Passive immunotherapy of viral infections: 'super-antibodies' enter the fray.
Walker et al. (2018)
17 more

7 mentions without citation

Single-Domain Antibodies As Versatile Affinity Reagents for Analytical and Diagnostic Applications.
Gonzalez-Sapienza et al. (2017)
6 more