› 5tpp

X-ray diffraction
1.85Å resolution

Crystal Structure of DH270.5 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage

Released:
DOI: 10.2210/pdb5tpp/pdb
Model geometry
Fit model/data
PDB entry 5tpp coloured by chain, front view.
PDB entry 5tpp coloured by chain, side view.
PDB entry 5tpp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Staged induction of HIV-1 glycan-dependent broadly neutralizing antibodies.
Bonsignori M, Kreider EF, Fera D, Meyerhoff RR, Bradley T, Wiehe K, Alam SM, Aussedat B, Walkowicz WE, Hwang KK, Saunders KO, Zhang R, Gladden MA, Monroe A, Kumar A, Xia SM, Cooper M, Louder MK, McKee K, Bailer RT, Pier BW, Jette CA, Kelsoe G, Williams WB, Morris L, Kappes J, Wagh K, Kamanga G, Cohen MS, Hraber PT, Montefiori DC, Trama A, Liao HX, Kepler TB, Moody MA, Gao F, Danishefsky SJ, Mascola JR, Shaw GM, Hahn BH, Harrison SC, Korber BT, Haynes BF
Sci Transl Med 9 (2017)
PMID: 28298420

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231431 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DH270.5 Fab heavy chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 22.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
DH270.5 Fab light chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 135.114Å b: 68.798Å c: 59.463Å
α: 90° β: 113.21° γ: 90°
R-values:
R R work R free
0.181 0.179 0.212
Expression system: Homo sapiens

Quick links

Citations

36 review citations

Transcription Factor NRF2 as a Therapeutic Target for Chronic Diseases: A Systems Medicine Approach.
Cuadrado et al. (2018)
35 more

2 mentions without citation

Staged induction of HIV-1 glycan-dependent broadly neutralizing antibodies.
Bonsignori et al. (2017)
1 more