› 5tqr

X-ray diffraction
2.57Å resolution

ctPRC2 in an autoinhibited conformation bound to S-adenosylmethionine

Released:
DOI: 10.2210/pdb5tqr/pdb
PDB entry 5tqr coloured by chain, front view.
PDB entry 5tqr coloured by chain, side view.
PDB entry 5tqr coloured by chain, top view.
Source organisms:
Primary publication:
Polycomb repressive complex 2 in an autoinhibited state.
Bratkowski M, Yang X, Liu X
J Biol Chem 292 13323-13332 (2017)
PMID: 28607149

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123899 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb Protein EED Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.02 KDa
Source organism: Thermochaetoides thermophila
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: G0S8H7 (Residues: 1-565; Coverage: 100%)
Gene name: CTHT_0029920
Sequence domains: WD domain, G-beta repeat
Polycomb protein VEFS-Box domain-containing protein; SET domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 937 amino acids
Theoretical weight: 106.87 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: G0SDW4 (Residues: 191-950; Coverage: 50%)
  • Canonical: G0RYC6 (Residues: 530-691; Coverage: 22%)
Gene names: CTHT_0006210, CTHT_0053230
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 117.454Å b: 138.008Å c: 223.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.233
Expression system: Saccharomyces cerevisiae

Quick links

Citations

6 review citations

Structure, mechanism, and regulation of polycomb-repressive complex 2.
Moritz et al. (2018)
5 more

2 mentions without citation

Engaging chromatin: PRC2 structure meets function.
Chammas et al. (2020)
1 more