› 5trl

X-ray diffraction
2.3Å resolution

Crystal structure of human GCN5 histone acetyltransferase domain

Released:
DOI: 10.2210/pdb5trl/pdb
PDB entry 5trl coloured by chain, front view.
PDB entry 5trl coloured by chain, side view.
PDB entry 5trl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase.
Wang Y, Guo YR, Liu K, Yin Z, Liu R, Xia Y, Tan L, Yang P, Lee JH, Li XJ, Hawke D, Zheng Y, Qian X, Lyu J, He J, Xing D, Tao YJ, Lu Z
Nature 552 273-277 (2017)
PMID: 29211711

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone acetyltransferase KAT2A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 168 amino acids
Theoretical weight: 19.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92830 (Residues: 497-662; Coverage: 20%)
Gene names: GCN5, GCN5L2, KAT2A
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand SCA 8 x SCA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P213
Unit cell:
a: 175.728Å b: 175.728Å c: 175.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

76 review citations

Regulation of chromatin and gene expression by metabolic enzymes and metabolites.
Li et al. (2018)
75 more

5 mentions without citation

Catalysis by protein acetyltransferase Gcn5.
Albaugh et al. (2021)
4 more