› 5tum

X-ray diffraction
3.3Å resolution

Crystal structure of tetracycline destructase Tet(56)

Released:
DOI: 10.2210/pdb5tum/pdb
PDB entry 5tum coloured by chain, front view.
PDB entry 5tum coloured by chain, side view.
PDB entry 5tum coloured by chain, top view.
Source organism: Legionella longbeachae
Primary publication:
Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.
Park J, Gasparrini AJ, Reck MR, Symister CT, Elliott JL, Vogel JP, Wencewicz TA, Dantas G, Tolia NH
Nat Chem Biol 13 730-736 (2017)
PMID: 28481346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 46.37 KDa
Source organism: Legionella longbeachae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3HKY4 (Residues: 1-389; Coverage: 100%)
Gene names: LLO_2673, tet(56)
Sequence domains: FAD binding domain

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21212
Unit cell:
a: 76.45Å b: 114.01Å c: 94.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.24 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Ribosome-Targeting Antibiotics: Modes of Action, Mechanisms of Resistance, and Implications for Drug Design.
Lin et al. (2018)
14 more

3 mentions without citation

Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.
Park et al. (2017)
2 more