› 5tx6

X-ray diffraction
2.75Å resolution

Structure of TGF-beta2 derivative with deletion of residues 52-71 and 10 single amino acid mutations (mmTGF-beta2-7M)

Released:
DOI: 10.2210/pdb5tx6/pdb
PDB entry 5tx6 coloured by chain, front view.
PDB entry 5tx6 coloured by chain, side view.
PDB entry 5tx6 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
An engineered transforming growth factor β (TGF-β) monomer that functions as a dominant negative to block TGF-β signaling.
Kim SK, Barron L, Hinck CS, Petrunak EM, Cano KE, Thangirala A, Iskra B, Brothers M, Vonberg M, Leal B, Richter B, Kodali R, Taylor AB, Du S, Barnes CO, Sulea T, Calero G, Hart PJ, Hart MJ, Demeler B, Hinck AP
J Biol Chem 292 7173-7188 (2017)
PMID: 28228478

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming growth factor beta-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 93 amino acids
Theoretical weight: 10.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27090 (Residues: 303-414; Coverage: 23%)
Gene name: Tgfb2
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 81.744Å b: 81.744Å c: 80.926Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.272
Expression system: Escherichia coli

Quick links

Citations

4 review citations

TGFβ biology in cancer progression and immunotherapy.
Derynck et al. (2021)
3 more

3 mentions without citation

An engineered transforming growth factor β (TGF-β) monomer that functions as a dominant negative to block TGF-β signaling.
Kim et al. (2017)
2 more