› 5txc

X-ray diffraction
2.4Å resolution

AtxE2 Isopeptidase - APO

Released:
DOI: 10.2210/pdb5txc/pdb
PDB entry 5txc coloured by chain, front view.
PDB entry 5txc coloured by chain, side view.
PDB entry 5txc coloured by chain, top view.
Source organism: Asticcacaulis excentricus CB 48
Primary publication:
Structure of the Lasso Peptide Isopeptidase Identifies a Topology for Processing Threaded Substrates.
Chekan JR, Koos JD, Zong C, Maksimov MO, Link AJ, Nair SK
J Am Chem Soc 138 16452-16458 (2016)
PMID: 27998080

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-123118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lasso peptide isopeptidase AtxE2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 705 amino acids
Theoretical weight: 78.7 KDa
Source organism: Asticcacaulis excentricus CB 48
Expression system: Escherichia coli
UniProt:
  • Canonical: E8RUP5 (Residues: 1-695; Coverage: 100%)
Gene names: Astex_2444, atxE2
Sequence domains: Prolyl oligopeptidase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C222
Unit cell:
a: 150.492Å b: 201.742Å c: 109.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Lasso Peptides: Heterologous Production and Potential Medical Application.
Cheng et al. (2020)
6 more

1 mention without citation

Structure of the Lasso Peptide Isopeptidase Identifies a Topology for Processing Threaded Substrates.
Chekan et al. (2016)