› 5ty5

Neutron Diffraction
2.3Å resolution

Neutron structure from microgravity-grown crystals of Inorganic Pyrophosphatase from Thermococcus theoreducens

Released:
DOI: 10.2210/pdb5ty5/pdb
PDB entry 5ty5 coloured by chain, front view.
PDB entry 5ty5 coloured by chain, side view.
PDB entry 5ty5 coloured by chain, top view.
Source organism: Thermococcus thioreducens
Primary publication:
Structure-function analysis of the neutron crystallographic structure of inorganic pyrophosphatase determined from microgravity-grown crystals
Inoguchi N, Coates L, Meilleur F, Morris ML, Singhal A, Barcena J, Garcia-Ruiz JM, Pusey ML, Ng JD
Acta Crystallogr., A, Found. Crystallogr. 73 132-132 (2017)

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 178 amino acids
Theoretical weight: 20.88 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand DOD 384 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: C2
Unit cell:
a: 105.93Å b: 95.03Å c: 113.5Å α: 90° β: 98.13° γ: 90°
R-values:
R R work R free 0.239 0.239 0.252
Expression system: Escherichia coli

Quick links