› 5u01

X-ray diffraction
2.5Å resolution

Cooperative DNA binding by two RelA dimers

Released:
DOI: 10.2210/pdb5u01/pdb
PDB entry 5u01 coloured by chain, front view.
PDB entry 5u01 coloured by chain, side view.
PDB entry 5u01 coloured by chain, top view.
Source organisms:
Primary publication:
DNA-binding affinity and transcriptional activity of the RelA homodimer of nuclear factor κB are not correlated.
Mulero MC, Huang DB, Nguyen HT, Wang VY, Li Y, Biswas T, Ghosh G
J Biol Chem 292 18821-18830 (2017)
PMID: 28935669

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119183 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription factor p65 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 273 amino acids
Theoretical weight: 31.01 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q04207 (Residues: 19-291; Coverage: 50%)
Gene names: Nfkb3, Rela
Sequence domains:
Structure domains:
DNA (27-MER) Chains: E, F
Molecule details ›
Chains: E, F
Length: 27 nucleotides
Theoretical weight: 8.29 KDa
Source organism: Synthesium
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 88.91Å b: 117.8Å c: 70.81Å
α: 90° β: 91.2° γ: 90°
R-values:
R R work R free
0.221 0.221 0.274
Expression system: Not provided

Quick links

Citations

4 review citations

Role of Silent Information Regulator 1 (SIRT1) in Regulating Oxidative Stress and Inflammation.
Singh et al. (2020)
3 more

21 mentions without citation

Susceptibility to amoxicillin-clavulanate-induced liver injury is influenced by multiple HLA class I and II alleles.
Lucena et al. (2011)
20 more