› 5u0a

Electron Microscopy
3.3Å resolution

CRISPR RNA-guided surveillance complex

Released:
DOI: 10.2210/pdb5u0a/pdb
PDB entry 5u0a coloured by chain, front view.
PDB entry 5u0a coloured by chain, side view.
PDB entry 5u0a coloured by chain, top view.
Source organism: Thermobifida fusca YX
Primary publication:
Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.
Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A
Cell 170 48-60.e11 (2017)
PMID: 28666122
Related structures: EMD-8478

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-119185 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein, Cse3 family Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.33 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ5 (Residues: 1-232; Coverage: 100%)
Gene name: Tfu_1588
Sequence domains: CRISPR associated protein
CRISPR-associated protein, Cse1 family Chain: C
Molecule details ›
Chain: C
Length: 549 amino acids
Theoretical weight: 61.43 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ1 (Residues: 1-549; Coverage: 100%)
Gene name: Tfu_1592
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR-associated protein, Cse4 family Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 373 amino acids
Theoretical weight: 41.04 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ3 (Residues: 1-373; Coverage: 100%)
Gene name: Tfu_1590
Sequence domains: CT1975-like protein
CRISPR-associated protein, Cse2 family Chains: J, L
Molecule details ›
Chains: J, L
Length: 244 amino acids
Theoretical weight: 27.45 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ2 (Residues: 1-244; Coverage: 100%)
Gene name: Tfu_1591
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
CRISPR-associated protein, Cas5e family Chain: N
Molecule details ›
Chain: N
Length: 254 amino acids
Theoretical weight: 28.28 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ4 (Residues: 1-254; Coverage: 100%)
Gene name: Tfu_1589
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Target Strand Chain: M
Molecule details ›
Chain: M
Length: 50 nucleotides
Theoretical weight: 15.25 KDa
Nontarget Strand Chain: O
Molecule details ›
Chain: O
Length: 39 nucleotides
Theoretical weight: 12.08 KDa
crRNA Chain: K
Molecule details ›
Chain: K
Length: 61 nucleotides
Theoretical weight: 19.79 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-8478
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Regulatory R-loops as facilitators of gene expression and genome stability.
Niehrs et al. (2020)
27 more

6 mentions without citation

The intrinsic ability of double-stranded DNA to carry out D-loop and R-loop formation.
Shibata et al. (2020)
5 more