› 5u1j

X-ray diffraction
2.95Å resolution

Structure of pNOB8 ParA bound to nonspecific DNA

Released:
DOI: 10.2210/pdb5u1j/pdb
PDB entry 5u1j coloured by chain, front view.
PDB entry 5u1j coloured by chain, side view.
PDB entry 5u1j coloured by chain, top view.
Source organisms:
Primary publication:
Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation.
Zhang H, Schumacher MA
Genes Dev 31 481-492 (2017)
PMID: 28373206

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119188 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
AAA domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 322 amino acids
Theoretical weight: 37.01 KDa
Source organism: Sulfolobus sp. NOB8H2
Expression system: Escherichia coli
UniProt:
  • Canonical: O93708 (Residues: 1-315; Coverage: 100%)
Sequence domains: AAA domain
DNA (5'-D(*TP*GP*AP*CP*GP*CP*CP*GP*GP*CP*GP*TP*CP*AP*TP*GP*AP*CP*AP*CP*G)-3') Chain: U
Molecule details ›
Chain: U
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*GP*TP*AP*AP*TP*GP*AP*CP*GP*CP*CP*GP*GP*CP*GP*TP*CP*A)-3') Chain: W
Molecule details ›
Chain: W
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 68.3Å b: 103.7Å c: 104Å
α: 90° β: 101.3° γ: 90°
R-values:
R R work R free
0.222 0.22 0.248
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Bacterial chromosome segregation by the ParABS system.
Jalal et al. (2020)
2 more

2 mentions without citation

Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation.
Zhang et al. (2017)
1 more