› 5u2h

X-ray diffraction
3.9Å resolution

Crystal structure of the ATP-gated P2X7 ion channel bound to ATP and allosteric antagonist A804598

Released:
DOI: 10.2210/pdb5u2h/pdb
PDB entry 5u2h coloured by chain, front view.
PDB entry 5u2h coloured by chain, side view.
PDB entry 5u2h coloured by chain, top view.
Source organism: Ailuropoda melanoleuca
Primary publication:
Structural basis for subtype-specific inhibition of the P2X7 receptor.
Karasawa A, Kawate T
Elife 5 (2016)
PMID: 27935479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ATP receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 38.72 KDa
Source organism: Ailuropoda melanoleuca
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: G1M6C4 (Residues: 24-359; Coverage: 55%)
Gene name: P2RX7
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand 7S1 2 x 7S1
Ligand NAG 1 x NAG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 167.618Å b: 167.618Å c: 167.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.337 0.334 0.386
Expression system: Spodoptera frugiperda

Quick links

Citations

45 review citations

The P2X7 Receptor in Infection and Inflammation.
Di Virgilio et al. (2017)
44 more

7 mentions without citation

Structural basis for subtype-specific inhibition of the P2X7 receptor.
Karasawa et al. (2016)
6 more