› 5u4h

X-ray diffraction
1.05Å resolution

1.05 Angstrom Resolution Crystal Structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Acinetobacter baumannii in Covalently Bound Complex with (2R)-2-(phosphonooxy)propanoic Acid.

Released:
DOI: 10.2210/pdb5u4h/pdb
PDB entry 5u4h coloured by chain, front view.
PDB entry 5u4h coloured by chain, side view.
PDB entry 5u4h coloured by chain, top view.
Source organism: Acinetobacter baumannii
Entry authors: Minasov G, Shuvalova L, Kiryukhina O, Dubrovska I, Grimshaw S, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 44.96 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R0VXX6 (Residues: 1-418; Coverage: 100%)
Gene names: A7M90_12815, ABR2091_0659, ABUW_3250, APD17_08525, AUO97_10600, B9W25_18125, B9X95_08860, CAS83_13370, CBE85_17835, CV954_016635, DOL94_15350, EA686_13295, EA706_17040, EA722_17610, F2P40_19240, F4T85_17000, FJU42_05650, G3N53_19280, GNY86_15415, GSE42_03835, IAG11_17070, IMO23_03230, JHZ39_003719, MKP18_003970, P9867_11855, SAMEA104305318_03048, SAMEA4394745_02172, murA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

3 bound ligands:
Ligand NA 9 x NA
Ligand FMT 4 x FMT
Ligand 0V5 2 x 0V5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 76.289Å b: 70.085Å c: 78.109Å
α: 90° β: 110.05° γ: 90°
R-values:
R R work R free
0.162 0.162 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links