› 5u4l

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230755 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.34 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:
V1C7 VHH antibody G29R variant Chain: B
Molecule details ›
Chain: B
Length: 139 amino acids
Theoretical weight: 15.14 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand CL 5 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 64.97Å b: 64.97Å c: 215.968Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.257
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Assessing human B cell repertoire diversity and convergence.
Imkeller et al. (2018)
11 other articles

2 mentions without citation

Identification of Cryptic Binding Sites Using MixMD with Standard and Accelerated Molecular Dynamics.
Smith et al. (2021)
1 more