› 5u5r

X-ray diffraction
2.1Å resolution

Crystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a PMS2 NLS Peptide

Released:
DOI: 10.2210/pdb5u5r/pdb
PDB entry 5u5r coloured by chain, front view.
PDB entry 5u5r coloured by chain, side view.
PDB entry 5u5r coloured by chain, top view.
Source organisms:
Primary publication:
DNA mismatch repair proteins MLH1 and PMS2 can be imported to the nucleus by a classical nuclear import pathway.
de Barros AC, Takeda AAS, Dreyer TR, Velazquez-Campoy A, Kobe B, Fontes MRM
Biochimie 146 87-96 (2018)
PMID: 29175432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156502 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Mismatch repair endonuclease PMS2 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P54278 (Residues: 573-583; Coverage: 1%)
Gene names: PMS2, PMSL2

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 77.124Å b: 89.544Å c: 97.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.207
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Genetic Polymorphisms of DNA Repair Pathways in Sporadic Colorectal Carcinogenesis.
Liu et al. (2019)
6 other articles