› 5u6m

X-ray diffraction
2.57Å resolution

Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and salicylic acid

Released:
DOI: 10.2210/pdb5u6m/pdb
Model geometry
Fit model/data
PDB entry 5u6m coloured by chain, front view.
PDB entry 5u6m coloured by chain, side view.
PDB entry 5u6m coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Differences in salicylic acid glucose conjugations by UGT74F1 and UGT74F2 from Arabidopsis thaliana.
George Thompson AM, Iancu CV, Neet KE, Dean JV, Choe JY
OpenAccess logo Sci Rep 7 46629 (2017)
PMID: 28425481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 74F2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 50.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22822 (Residues: 1-449; Coverage: 100%)
Gene names: At2g43820, F18O19.7, GT, SAGT1, SGT1, UGT74F2
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand BGC 2 x BGC
Ligand UDP 2 x UDP
Ligand SAL 2 x SAL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 65.694Å b: 87.681Å c: 164.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.26
Expression system: Escherichia coli

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Citations

5 review citations

Salicylic Acid Binding Proteins (SABPs): The Hidden Forefront of Salicylic Acid Signalling.
Pokotylo et al. (2019)
4 more

7 mentions without citation

Structure-function and engineering of plant UDP-glycosyltransferase.
Wang et al. (2023)
6 more