› 5ua1

X-ray diffraction
2.9Å resolution

Mycobacterium tuberculosis KstR in complex with a 18-bp DNA operator

Released:
DOI: 10.2210/pdb5ua1/pdb
PDB entry 5ua1 coloured by chain, front view.
PDB entry 5ua1 coloured by chain, side view.
PDB entry 5ua1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Ho NAT, Dawes SS, Baker EN, Lott JS, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119202 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional repressor KstR Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.35 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96856 (Residues: 20-220; Coverage: 91%)
Gene names: Rv3574, kstR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*AP*CP*TP*AP*GP*AP*AP*CP*GP*TP*GP*TP*TP*CP*TP*AP*AP*T)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
DNA (5'-D(*AP*TP*TP*AP*GP*AP*AP*CP*AP*CP*GP*TP*TP*CP*TP*AP*GP*T)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 61.423Å b: 79.28Å c: 116.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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