› 5ucy

Electron Microscopy
4.6Å resolution

Cryo-EM map of protofilament of microtubule doublet

Released:
DOI: 10.2210/pdb5ucy/pdb
PDB entry 5ucy coloured by chain, front view.
PDB entry 5ucy coloured by chain, side view.
PDB entry 5ucy coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins.
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun 8 15035 (2017)
PMID: 28462916
Related structures: EMD-8539

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154382 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 48.79 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: P41351 (Residues: 1-441; Coverage: 98%)
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 429 amino acids
Theoretical weight: 48.01 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: P41352 (Residues: 1-429; Coverage: 97%)
Gene names: BTU1, BTU2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-8539

Quick links

Citations

10 review citations

A microtubule bestiary: structural diversity in tubulin polymers.
Chaaban et al. (2017)
9 more

1 mention without citation

Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins.
Ichikawa et al. (2017)