Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
DNA-directed RNA polymerase subunit beta' (preferred)
PDBe Complex ID:
PDB-CPX-161530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains: RNA polymerase Rpb1, domain 1
Length: 169 amino acids
Theoretical weight: 19.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical:
P9WGY7 (Residues: 124-271; Coverage: 11%)
Sequence domains: RNA polymerase Rpb1, domain 1
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 19-ID
Spacegroup:
P21
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
