› 5uho

X-ray diffraction
3.21Å resolution

Crystal structure of the core catalytic domain of human O-GlcNAcase complexed with PUGNAc

Released:
DOI: 10.2210/pdb5uho/pdb
PDB entry 5uho coloured by chain, front view.
PDB entry 5uho coloured by chain, side view.
PDB entry 5uho coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Klein DJ, Elsen NL

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein O-GlcNAcase Chains: A, C
Molecule details ›
Chains: A, C
Length: 345 amino acids
Theoretical weight: 39.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60502 (Residues: 56-400; Coverage: 38%)
Gene names: HEXC, KIAA0679, MEA5, MGEA5, OGA
Sequence domains: beta-N-acetylglucosaminidase
Structure domains: Glycosidases
Protein O-GlcNAcase Chains: B, D
Molecule details ›
Chains: B, D
Length: 163 amino acids
Theoretical weight: 18.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60502 (Residues: 544-705; Coverage: 18%)
Gene names: HEXC, KIAA0679, MEA5, MGEA5, OGA

Ligands and Environments

1 bound ligand:
Ligand OAN 2 x OAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P41212
Unit cell:
a: 97.34Å b: 97.34Å c: 260.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.259
Expression system: Spodoptera frugiperda

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