› 5uhs

X-ray diffraction
2.8Å resolution

Structure of a SemiSWEET D57A mutant

Released:
DOI: 10.2210/pdb5uhs/pdb
PDB entry 5uhs coloured by chain, front view.
PDB entry 5uhs coloured by chain, side view.
PDB entry 5uhs coloured by chain, top view.
Source organism: Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
Primary publication:
Mechanism of Substrate Translocation in an Alternating Access Transporter.
Latorraca NR, Fastman NM, Venkatakrishnan AJ, Frommer WB, Dror RO, Feng L
Cell 169 96-107.e12 (2017)
PMID: 28340354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar transporter SemiSWEET Chains: A, B
Molecule details ›
Chains: A, B
Length: 93 amino acids
Theoretical weight: 10.68 KDa
Source organism: Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
Expression system: Escherichia coli
UniProt:
  • Canonical: B0SR19 (Residues: 1-85; Coverage: 100%)
Gene name: LEPBI_I1613
Sequence domains: PQ loop repeat
Structure domains: Monooxygenase

Ligands and Environments

2 bound ligands:
Ligand MVC 2 x MVC
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 47.021Å b: 36.92Å c: 47.514Å
α: 90° β: 104.27° γ: 90°
R-values:
R R work R free
0.246 0.242 0.268
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
15 more

1 mention without citation

Mechanism of Substrate Translocation in an Alternating Access Transporter.
Latorraca et al. (2017)