5ui5

X-ray diffraction
3.4Å resolution

Crystal structure of Aquifex aeolicus sigmaN bound to promoter DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119221 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RNA polymerase sigma factor RpoN Chains: I, V
Molecule details ›
Chains: I, V
Length: 342 amino acids
Theoretical weight: 40.34 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66858 (Residues: 61-398; Coverage: 85%)
Gene names: aq_599, rpoN
Sequence domains:
Structure domains:
DNA (31-MER) Chains: A, N
Molecule details ›
Chains: A, N
Length: 31 nucleotides
Theoretical weight: 9.63 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided
DNA (30-MER) Chains: B, O
Molecule details ›
Chains: B, O
Length: 31 nucleotides
Theoretical weight: 9.47 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, NSLS BEAMLINE X3A
Spacegroup: P32
Unit cell:
a: 99.921Å b: 99.921Å c: 123.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.289 0.336
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided