› 5ujy

X-ray diffraction
2.7Å resolution

The structure of Mycobacterium tuberculosis topoisomerase I from the 2nd crystal form

Released:
DOI: 10.2210/pdb5ujy/pdb
PDB entry 5ujy coloured by chain, front view.
PDB entry 5ujy coloured by chain, side view.
PDB entry 5ujy coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site.
Cao N, Tan K, Annamalai T, Joachimiak A, Tse-Dinh YC
Nucleic Acids Res 46 7296-7308 (2018)
PMID: 29905859

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 706 amino acids
Theoretical weight: 77.84 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG49 (Residues: 2-704; Coverage: 75%)
Gene names: MTCY15C10.06, Rv3646c, topA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 170.573Å b: 64.412Å c: 169.345Å
α: 90° β: 112.24° γ: 90°
R-values:
R R work R free
0.2 0.198 0.243
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanism of Type IA Topoisomerases.
Dasgupta et al. (2020)
3 more

1 mention without citation

Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site.
Cao et al. (2018)