› 5uk7

X-ray diffraction
3Å resolution

Escherichia coli Hfq bound to dsDNA

Released:
DOI: 10.2210/pdb5uk7/pdb
PDB entry 5uk7 coloured by chain, front view.
PDB entry 5uk7 coloured by chain, side view.
PDB entry 5uk7 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Orans J, Kovach AR, Brennan RG

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119222 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RNA-binding protein Hfq Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 68 amino acids
Theoretical weight: 7.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6X3 (Residues: 2-69; Coverage: 67%)
Gene names: JW4130, b4172, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.
DNA (5'-D(P*CP*GP*GP*CP*AP*AP*AP*AP*AP*AP*CP*GP*GP*CP*AP*AP*AP*AP*AP*A)-3') Chains: N, Z
Molecule details ›
Chains: N, Z
Length: 20 nucleotides
Theoretical weight: 6.19 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*GP*CP*CP*GP*TP*TP*TP*TP*TP*TP*GP*CP*CP*G)-3') Chains: M, Y
Molecule details ›
Chains: M, Y
Length: 20 nucleotides
Theoretical weight: 6.08 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 65.749Å b: 65.795Å c: 81.996Å
α: 105.93° β: 92.28° γ: 119.92°
R-values:
R R work R free
0.213 0.21 0.26
Expression systems:
  • Escherichia coli
  • Not provided

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